Muhammad Asfandyar Khan

4 Questions 1 Answers 0 Followers

Questions related from Muhammad Asfandyar Khan

Alternative to horizontal vortex adapter for efficient bead beating to extract the soil microbiome using the PureLink™ Microbiome?

I don't have a horizontal adapter for vortexing, which is required for the bead beating process according to the protocol. Is there any alternative? Will using normal vertical vortexing work? Can...

10 May 2023 8,217 1 View

For how long I can store the water sample at 4 degree centigrade for bacterial culture analysis?

I have a very short window of collecting the water samples (50ml) and wont be able to culture them for a month. For how long I can store them and and is there anyway to increase the storage time.

29 November 2022 8,271 4 View

How to Primer design for the detection Antimicrobial resistance gene through qPCR/Real timePCR?

I want to extract TEM or blaTEM gene of E Coli for QPCR. But first I have to get the sequence of them gene followed by primer design and then In Silico PCR after that. Can someone tell me where...

25 October 2022 330 2 View

Is there any way to recover bacteria from a frozen water sample?

One of my junior colleagues froze a water sample (20ml) at a temperature of -2 degrees Celsius without adding glycerol. Now, you're wondering if there is a method to quantify the bacterial load in...

01 January 1970 5,909 2 View