How to prevent the tissue from peeling off the slides when using frozen sections for IHC?

06 June 2013 1 7K Report

I'm using frozen sections to quantify T lymphocytes by using immunohistochemistry. After incubations with H2O2, we have tried 3% and.3%, I have lost most of the tissue. Has anybody experienced this and solved it?

Michael Tighe

Try Silane coated slides

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