Hello everyone,
Hope all are doing good.
I would like to know how they prevent contamination in producing FBS at the moment.
I am looking for methods for preventing contamination, without using high temperatures or antibiotics.
Thanks
Hello Asghar Mohammadzadeasl
You can use gamma irradiation (≥25 kGy) which is one of the most widely used and effective methods for the inactivation of viruses and mycoplasmas in animal sera. Gamma irradiation can inactivate the viruses commonly present in bovine species. However, highly resistant parvoviruses, caliciviruses, and polyomaviruses may require higher than normal doses of radiation to avoid contamination.
Another method is filtration. FBS product is triple filtered through 0.1μm filters, mainly targeting mycoplasma and other larger microbial contaminants. Recently, more advanced barrier virus removal filters have been introduced, like the Viresolve Barrier Filters. In the case of serum filtration, it is extremely challenging to filter such a highly complex protein mixture through filters, since these filters rapidly foul during processing. To avoid fouling, it is common to introduce multiple prefiltration steps to exclude coarse aggregates. However, the drawback of the latter approach is that every prefiltration step may lead to unwanted product losses due to nonspecific adsorption of media components.
Best.
Hello,
By nanofiltration, but a significant proportion of proteins (about 45%) will be eliminated. Most importantly, transport proteins such as albumin, fetuin, macroglobulin, and transferrin will not be affected. When cultivating in nanofiltered FBS, very promising results in cell viability were obtained, according to this study ( doi: 10.1007/s10616-013-9633-8).
Best regards
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