When I put spin column in opened eppendorf tube to elute DNA, I lost the eppendorf cups which are mostly breaking through this process, so I anyone have an experience that help to avoid this problem please.
Better to reduce the speed of the rotor. In my case, generally I spin the column at 8,000-10,000 rpm to elute DNA and my eppendorf cups remains intact. I experienced same kind of problem as yours when I went for rotor speed of more than 10,000 rpm.
To elute DNA with spin column, a speed of 8000-10000 rpm is more than sufficient.
Depending on the rotor of choice kindly calculate the RCF values appropriate for the elution process.
You can also use vacuum based methods for elution instead of centrifugation.
Hi there,
To avoid the caps to break I only load half of the rotor leaving one empty hole between samples. I adjust the caps above the empty holes trying to flaten them so that with centrifugal force the cap movement will be limited by the rotor cover. I also respect orientation of the sample towards revolution : tube body lying ahead in front of the cap. I have experienced cap breakage only when the rotor was fully occupied meaning there was no room to adjust the caps properly in the rotor.
Thank you Dominique, Jay and Manoj. In appreciate your help in fact I tried today to place the cups to the out of rotor and tilted them with rotor movement direction which was worked perfectly at high speed 14000 xg.
It's a bit late to answer, but maybe it will help somebody. You can cross the caps (as shown at the picture) with each other, that way you can load even full centrifuge and spin at the max speed, and caps wont break👍
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