Laila Ziko

6 Questions 3 Answers 0 Followers

Questions related from Laila Ziko

Is there a mobile phase to separate acetyl-coA, malonyl co-A from free co-enzyme A on TLC? Thank you!

I need to separate acetyl co-A, malonyl co-A & free co-enzyme A on TLC, I tried several mobile phases but they didn't work with acetyl co-A, it didn't move. I tried: ethyl acetate, methanol...

07 July 2017 3,567 1 View

What is the best method to sequence Fosmid DNA?

Hi, I have a fosmid of size ~40 kb, what would be the best method to sequence it & capture all the insert?

10 October 2016 8,185 3 View

How can I extract fosmid DNA without getting multiple bands?

I need to send Fosmid DNA for sequencing, but they told me the concentrations were too low & there are multiple bands. I tried mini-prep Qiagen kit many times & also midi-prep. How can...

08 August 2016 8,115 2 View

Is this a protein extract protocol? ptns vs. metabolites?

(Felczykowska et al., 2014): Overnight cultures from E. coli were centrifuged at 3,500 rpm for 10 min. Afterwards, the cell pellets were re-suspended in 0.01 M Tris-HCl pH 7. Finally the extracts...

06 June 2016 2,499 0 View

How to preserve the precipitates of an MTT assay if I'm not going to read the plate immediately?

What if I don't read the plate immediately, what should I do and how do I preserve the plate? in the fridge or on the bench? and is it reliable to do so?

12 December 2013 5,613 2 View

Using bbduk for decontaminating sequences? I did Mi-seq for fosmid DNA but got some E. coli sequences contaminating the data, how can I remove them?

Using bbduk for decontaminating sequences? I did Mi-seq for fosmid DNA but got some E. coli sequences contaminating the data, how can I remove them? Someone recommended I use bbduk for this, but I...

01 January 1970 2,378 0 View