How to preserve Alexa Fluor–labeled gold nanoparticles in mouse tumor tissue for confocal microscopy?

08 July 2025 0 8K Report

I am working with gold nanoparticles tagged with Alexa Fluor dyes and need to injected them into mouse tumors. I would like to visualize their internalization and distribution within the tumor tissue using confocal microscopy.

Could anyone please advise on:

The best way to preserve tumor tissue post-injection to retain Alexa Fluor signal?

Whether paraformaldehyde (PFA) fixation (e.g., 4% PFA perfusion and post-fixation) affects the fluorescence intensity or stability of Alexa Fluor dyes in tissue, especially when conjugated to nanoparticles?

If anyone has experience with similar nanoparticle imaging protocols (e.g., fixation time, cryoprotection, or sectioning method), I would greatly appreciate your input.

Thank you!

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