I still have no experience in preparing WPM medium. I devided mineral salt component into stock 1, stock 2 and stock 3 that are macronutrients, Na2EDTA&FeSO4.7H2O and micronutrients, respectively. In the preparation of stock 1, I tried to separate CaCl2,CaN03.4H2O and MgSO4.7H20, NH4NO3, KH2PO4, KNO3 into 2 solutions which are diluted completely. However, when I mixed 2 solutions together and stired them, then white precipation appeared after 2 minutes. I tried to repeat three times but there is no difference in result. I hope I could be given some experienced advices.
Dear Thắng Nguyễn Phước Quý I always prepare stocks separating nitrates from sulphates. In this way, it works. However, you must to check the stock concentration. For macronutrients, 20X is the most "suitable". In some cases, depending on formulation, 50X stock solutions could be also prepared
Avoid preparation of concentrated solutions, as it will lead to precipitation of salts. Also, Concentrated solutions should not be added to an empty beaker or with a small amount of water, as this will lead to precipitation of mineral salts. For the same reason, you should stir vigorously when adding concentrated solutions.
good luck..................regards
olha, eu trabalhei com ficcus benjamina varegatano meu estagio e nao tive problemas em relação a precipitação. porem a"sempre a qualidade doa materia prima interfere" me respponde algo, com que especie vc esta trabalhando? esta fazendo indução hormonal?
I am so grateful for your help. I finally prepared WPM medium.