before the starting of FRAP assay it needs to be preparation like homogenization or fractionations. my question is exactly focused on the homogenization methods. if there is any other technique, let me know.
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How to use ERP System in Global University ?
27 July 2024 8,229 0 View
here's a concise guide for preparing your CSV dataset in Excel to identify flood-triggering factors using an ANN: Clean and Format:Address missing values (fill with mean/median or remove if...
21 June 2024 7,941 0 View
how do integrate ECG, PCG, and clinical data to apply early fusion multimodal?
10 June 2024 3,289 1 View
I have performed the Molecular Docking using ADFR suite, I have obtained the files in _out.pdbqt format for ligand docked poses, upon inspection, both visually and reading the pdbqt files of...
08 June 2024 8,130 2 View
Dear Respected Scholars, I am working on detection of microplastic in prawn. Although, all the other parameters assessed smoothly but I have encounterd a problem during isolation of microplastics...
05 June 2024 2,469 0 View
In basin delineation and hydrological modeling, selecting the exact value in Flow Accumulation to identify streams is a critical step. The Flow Accumulation tool calculates the accumulated flow as...
30 May 2024 6,107 1 View
Are multi-level models appropriate for binary outcome variables for DHS datasets? If yes, what is the process?
30 May 2024 2,035 2 View
For my project, I need to extract RNA from muscle tissue of EEL. I am trying but the yield is low and Purity not good. The 260:230 ratio is always below 1.50 and 260:280 ratio ranges 1.6-1.86 and...
15 May 2024 3,024 2 View
For the design of three phase inverter for three phase motor drive, which is better using low DC voltage (12 V or 24 V DC) for the inverter stage then step it up using three phase transformer, or...
08 May 2024 3,491 3 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.
17 July 2024 3,532 0 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?
15 July 2024 1,598 3 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View
Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...
10 July 2024 6,830 1 View
Hi, I would appreciate professional and technical insights regarding primary cell culturing. I've been working on optimizing my primary cell culture, and while it is successful at times, there are...
10 July 2024 4,918 2 View
Hello everyone, I'm working with a PA-1 ovarian cancer cell line xenograft model in female nude mice. My cells are at 372 passages, the cell repository bank themselves gave at 352th passage....
05 July 2024 5,860 1 View