Can anyone explain me how to prepare this standard solution step by step or refer me some paper where each step is explained.
My doubt is how to dilute different concentrations for TEP ? by ethanol?
It's soluble in water. At 25°C, its 4.17 M, so 10 ul + 407 ul water gives a 100 mM solution.
Genome size is avilable at NCBI website, but for size of a particular gene of Aedes aegypti where should one look for. For example nix and fru geens of Aedes aegypti
11 September 2023 9,346 0 View
the proteins are known to interact and form a heterodimer and a possible tetramer. There is no structural information available for the same. I have tried predicting the structure through google...
24 July 2023 9,050 0 View
I am trying to perform optimization studies for which I want to use RSM but my concern is to which design to use for my study like there is central composite, Box behennken, etc. Kindly help me...
22 June 2023 2,083 1 View
I'm performing iodine value test to find the unsaturation present in the oil. but I am facing problem at the endpoint is colorless. I'm not getting what is wrong with the wijs solution that I'm...
21 June 2023 2,842 0 View
How to obtain Relative humidity 75% using NaCl ? How much Nacl needs to be dissolved in water in order to make saturated soltuion?
01 May 2023 9,495 2 View
I want to diagnose my mutant plants. So I am looking for an easy method for diagnosing the chromosome.
28 August 2022 8,782 0 View
I want to find SOD and MBD from pomegranate leaves. Is it possible to carryout experiment with fresh leaf samples?
22 June 2022 3,041 5 View
I have been trying to extract anthocyanin using different concentrations of alcohol. For that TPC, TAC and Antioxidant activity (DPPH method) are the following analysis that I'm performing. So,...
14 March 2022 9,088 10 View
Hello Research fellows, I need to design some figures for my project. Can anyone please suggest some free software or tools for the same other than MSpowerpoint/publisher? Thanks in advance
03 March 2022 5,862 5 View
I'm performing the DPPH Assay for my plant extract. So with an increase in the conc of extract (20,40,60,80,100), either the % is decreasing or showing an irregular pattern. Also, it is showing...
20 February 2022 4,067 13 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.
17 July 2024 3,532 0 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?
15 July 2024 1,598 3 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View
Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...
10 July 2024 6,830 1 View
Hi, I would appreciate professional and technical insights regarding primary cell culturing. I've been working on optimizing my primary cell culture, and while it is successful at times, there are...
10 July 2024 4,918 2 View
Hello everyone, I'm working with a PA-1 ovarian cancer cell line xenograft model in female nude mice. My cells are at 372 passages, the cell repository bank themselves gave at 352th passage....
05 July 2024 5,860 1 View