I have soil samples and i want to conducts some microbiological principles, what are principles and Sources of Error
There is no special preparation adopted for soil sample preparation for microbiological analysis , except that samples have to be dried in shade and stored in cool place . And analysis should be performed as early as possible after soil sampling .
Your question is not so clear. Whether you want to do screening for some specific study or you want to identify the microbes in that sample ?
If you are looking to screen some microbes for your purpose, then collect the soil sample and mix thoroughly with phosphate buffer or saline with pH 7 and enrich the microbes in some general media and then streak the sample on media (for bacteria- PCA , for fungus- SDA or YEGCA) and then after the incubation time select the isolated colonies and then continue your studies
What I have learnt, mostly from reading articles, is that keeping the soil samples as cool as possible after collection (around 4 degrees Celsius) is a good practice. Then do whatever measurements you have to as soon as possible. The fresher is always the better.
There is no special preparation adopted for soil sample preparation for microbiological analysis , except that samples have to be dried in shade and stored in cool place . And analysis should be performed as early as possible after soil sampling .
Tell us the parameters which you are looking for and there are specific preparations for the same.
I have very sandy soils. I do not know what I am looking for, but for now I would like to see if I can find differences in the soil where these indigenous plants grow and where they do not grow in the same area. Rooibos tea
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