What is the extraction methods for growth substances in nitrogen fixing cyanobacteria Anabaena sp.?

Abdelgawad Y. Elsadany @Abdelgawad_Elsadany

02 February 2016 1 3K Report

I want to extract growth substances such as gibberellins and Auxins from nitrogen fixing cyanobacteria Anabaena sp..

we have done this work as part of M.Sc. thesis by one of my students. the procedure is to grow the cyanobacteria in pure culture and then extract a known volume of the pure culture with known volume of 80% methanol three times., and then follow the extraction procedure with partitioning with diethyl ether after adjusting the PH of the extractto 3.5. then reducing the volume of the extract to 1ml using rotary evaporator at 45C.then use HPLC for the determination of gibberellins and IAA.. the thesis is written in Arabic, and it published in arabic in BASRAH J.AGRIC. SCI,1998.

Badges
Science topic

More Abdelgawad Y. Elsadany's questions See All

Why the orange color does not appear when the Dunaliella microalga is exposed to stress conditions?

When growing the Dunaliella microalga under stress conditions in the laboratory such as nitrogen deficiency and high salinity in order to extract beta-carotene. It does not change the color from...

11 December 2017 9,661 1 View

What kind of toxins produced by the toxin cyanobacterium Nodularia harveyana and factors affecting its production?

01 February 2016 324 1 View

Why add 10 gram agar per liter to medium solid of cyanobacteria?

31 December 2015 9,561 4 View

How to prepare soil samples for microbiological analysis?

31 December 2015 9,299 6 View

Any one has idea of levan sucrase from halophilic bacteria

microbial polysaccharide, halophilic bacteria

07 August 2014 5,377 0 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View