Suppose I have 100 microlitre of human blood. How to prepare samples of 10 RBC/microlitre, 20 RBC/microlitre, 50 RBC/microlitre, and 100 RBC/microlitre of the collected blood?
Hi Jalal,
It is quite straigthforward once you know the red blood cell count, either in your tested blood sample or washed red blood cells. Either way you can count the cells using hemocytometer. When working with a whole blood sample, dilute it 200 times before applying onto a chamber, to facilitate counting. For packed RBCs, start from 500 times. Remember the dilution factor for the final calculation.
Good luck,
Radek
Actually, I am working on colorimetric analysis of urine analyte. In my analysis, I need to prepare samples of 10, 20, 50 and 100 RBC per micoliter from from collected blood to react with urine test strip and study its color change for further analysis. We do not have hematocytometer or any hematology analyzer. Is there any straight forward method to prepare those RBC samples from the collected blood?
Without knowing the cell numbers counted either with an automated system or manual hematocytometer, you will not be able to make an exact dilution..
What do you mean you don't have a hemocytometer? Gulderen is right, you don't need any state-of-the-art equipment. Burker's chamber is as straightforward as it gets.
Good luck,
Radek
As i have described in your previous question answer, centrifuge, dilute , count by hemocytometer and separate your desired number of RBC.
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