How to prepare pH = 8.6 tris acetate?

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María Luisa Caballero

Do you mean TAE Buffer?

If you mean the TAE Buffer (0.04 M, pH 8.5), you can prepare a 50X stock solution as follow: 242 g Tris base in 800 ml water, then add 57.1 ml glacial acetic acid, and 18.61 g of disodium EDTA to the solution. Finally, add water bringing the final volume up to 1 L.