Srdjan Masirevic

2 Questions 3 Answers 0 Followers

Questions related from Srdjan Masirevic

How to prepare pH = 8.6 tris acetate?

I am trying to perform in vitro binding assay and in one of my assay mix I have to use tris acetate at pH od 8.6. Since I already have a Tris as a powder and acetate as liquid, how can I prepare it?

01 January 2020 6,808 2 View

Structural difference of X-ray and NMR solved protein structures (same protein) comes from the dynamic nature or specific exp conditions (Temp, pH)?

If there is a structural difference that exists in the backbone and side chain dihedral angles etc. between X-ray and NMR structure of the same protein, how do we know if this difference is due...

09 September 2019 3,751 2 View