I need to conduct adsorption studies on Vanadium using Biochars, nanoxides and some other soil amendments . To prepare Vanadium solutions up to 320 ppm with a pH buffered at 7.5 using PIPES buffer, how should I proceed? Yesterday, I used 10 mM PIPES and adjusted pH to 7.5-7.55 for concentrations up to 40 ppm. However, at 80 ppm, pH rose to 11. Adding a few microliters of 1N HNO3 turned the solution yellow, possibly due to deca vanadic acid formation. We have limited buffer and prefer to use orthovanadate only. Any suggestions?"
Add powder Sigma, St. Louis, MO to 30 mL H2O and adjust to pH 7.5 with HCl. The volume is adjusted and the solution is stored at 4°C for up to two weeks and then at room temp.
To prepare vanadium solutions up to 320 ppm buffered at pH 7.5, use a higher concentration of PIPES buffer (20-50 mM) and sodium orthovanadate, adjusting pH carefully with dilute acids. Avoid concentrated HNO3 to prevent decavanadic acid formation, and prepare fresh solutions for consistent results.
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