For studying antioxydant activities , many protocols use dry matter such as leaves or roots to prepare methanolic extract..My question is what about using fresh matters ? Can we use fresh material to prepare methanolic solutions ? Thanks.
Yes. You can use Fresh material to make methanolic extract. Keep in mind that fresh material contains water and it will dilute your methanol percentage.
Dear
The use of dry samples is common for reasons, fresh samples contain high levels of moisture which not active ingredient, and also lowering the percentage yield. The use of fresh samples means that you try to detect compounds will degrade before samples dry. However antioxidant compounds are usually phenolic compounds and most of them gained well with dry samples extraction.
Regards
Fresh sample can be taken, however, it may dilute the concentration of solvent.
If you are intended to use alcohol-water mix, as an extractant for fresh sample, be mindful to use higher ratio (70:20 or more) to nullify the dilution effect.
Dry samples are better for concentrated extract with maximum of biocompounactives quantitively & qualitatively
Dear Dr Maher,
It is not acceptable to use fresh material of leaves for extraction apart to be dried material. Let us ask ourselves, how much water in that leaves, how will you belong the expression to the starting material, basically how to extract a material you do not have any experimental knowledge about it for example, % of water or dry matter. Please find my guide to you for better extraction in aqueous methanol.
Good luck
Aly
Thesis Chapter 2-TO INVESTIGATE THE ANTIOXIDANT PROPERTIES OF DIETA...
Dear Dr Maher
Please find how to cite the reference above
A.R. Abdel-Moemin, To investigate the antioxidant properties of dietary flavonoids in human metabolism. PhD Thesis, The Queen's University of Belfast, United Kingdom, 2004.
Following the folk medicine, the plants are sometimes used in fresh form, or sometime dried plants are necessary. In the fresh plant the glycosylated compounds are more abundant and they undergo hydrolysis by drying.In plants used as food is preferred the fresh extract.
You can use fresh material.Better use dry samples.for quantitative determination. In fresh samples you may have solvent dilution.
how can we make dry powder of ethanolic extacts? Every time during drying we get semi-solid or paste like material.
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