Hello, here the ingredients for lactophenol cotton blue

1.Cotton Blue (Aniline Blue) 0.05 g

2.Phenol Crystals  20 g

3.Glycerol 40 mL

4.Lactic acid  20 mL

5.Distilled water 20 mL

for preparation you need to use lactic acid 85% solution

Good luck!

1umol/l=0,09mg/l =0,09ug /ml

Hello, here the ingredients for lactophenol cotton blue

1.Cotton Blue (Aniline Blue) 0.05 g

2.Phenol Crystals  20 g

3.Glycerol 40 mL

4.Lactic acid  20 mL

5.Distilled water 20 mL

for preparation you need to use lactic acid 85% solution

Good luck!

Hello,

Please see the following link, since it is exactly the same procedure that is use my lab (and by those that already aswered )

https://mycology.adelaide.edu.au/laboratory/lacto/

First of all I fail to understand : How did you get solid lactic acid.I feel it should be some kind of salt. Read the label carefully.

Next point is to prepare lacto-phenol-blue : Karen has given the right answer for it.