I want to prepare lactophenol cotton blue for fungi staining. But I have solid lactic acid. The protocol said to use 20 ml of lactic acid solution. question is -
1. What should be the percentage of the lactic acid in solution?
2. How to make it?
Hello, here the ingredients for lactophenol cotton blue
1.Cotton Blue (Aniline Blue) 0.05 g
2.Phenol Crystals 20 g
3.Glycerol 40 mL
4.Lactic acid 20 mL
5.Distilled water 20 mL
for preparation you need to use lactic acid 85% solution
Good luck!
1umol/l=0,09mg/l =0,09ug /ml
Hello,
Please see the following link, since it is exactly the same procedure that is use my lab (and by those that already aswered )
https://mycology.adelaide.edu.au/laboratory/lacto/
First of all I fail to understand : How did you get solid lactic acid.I feel it should be some kind of salt. Read the label carefully.
Next point is to prepare lacto-phenol-blue : Karen has given the right answer for it.
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