I need to make 3.8% (w/v) solution Fe-EDTA and 0.1% (w/v) NaMo•4H2O for NMS media.
1. So far I've seen most protocols use Na2EDTA.2H2O + Fe(NH4)2(SO4)2•6H2O to make Fe-EDTA. One from Garcia-Pirchel's lab website uses Na2EDTA•2H2O + FeSO4•7H2O + KOH. I have FeSO4•7H2O and KOH in the lab and plan to use this way. I don't have Na2EDTA but I do have EDTA. Which brings me to my question: can I use EDTA for the mentioned recipe?
2. 0.1% (w/v) NaMo•4H2O - can someone let me know how can I make this? I have Sodium molybdate and sodium molybdate (VI) dihydrate in the lab. Can these be used to prepare the 0.1% (w/v) NaMo•4H2O ? I can't seem to find any protocols for this specific reagent.
Thank you in advanced.
While I don't see what's your plan, I'd say:
1. Yes, should be possible. Maybe it's necessary to adjust the pH as EDTA is more acidic than its sodium form.
2. Never heard of NaMo. Sodium molybdate is Na2MoO4. There is a dihydrate and an anhydrous form. In the end, it should be about the Mo concentration. So, calculate this accordingly.
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