Jasmine Keller

5 Questions 5 Answers 0 Followers

Questions related from Jasmine Keller

How to increase qPCR efficiency to acceptable range?

My aim is to create a standard for qPCR for absolute quantification of DNA extracted from soil. The reaction contains PowerUp SYBR Green Master Mix, BSA, primer pair (A189F-MB661R- 0.67 uM), and...

11 June 2024 6,579 9 View

How to convert ng to ul of PCR product for ligation of pGEM-T Easy?

As suggested by the formula by Promega's pGEM-T Easy protocol, (ng of vector x kb size of insert)/kb size of vector x insert:vector molar ratio= ng of insert using example given by protocol,...

13 September 2023 5,513 1 View

How to calculate concentration using external standard (GC FID)?

I sampled the headspace of my incubation gas, and target the CH4 compound. How do I determine its concentration in unit of "umol g dw soil^-1" ? I have plotted the calibration curve and obtained...

11 August 2023 4,502 1 View

How to calculate gas phase for culturing methanotroph (Mineral medium)?

1. I am trying to culture methanotrophs using the mineral medium (DSMZ 1007 Mineral Medium) . The instruction says to grow it under gas phase of 20% methane in air. By culturing this using bottle...

01 March 2023 1,182 5 View

How to prepare Fe-EDTA & NaMo•4H2O ?

I need to make 3.8% (w/v) solution Fe-EDTA and 0.1% (w/v) NaMo•4H2O for NMS media. 1. So far I've seen most protocols use Na2EDTA.2H2O + Fe(NH4)2(SO4)2•6H2O to make Fe-EDTA. One from...

16 February 2023 7,557 1 View