Why did I obtain abnormal staining by TUNEL assay?

I use Abcam TUNEL Assay Kit - HRP-DAB (ab206386) on mouse tissues. The staining showed a pattern that I expected to see with a specific layer of cells dying due to drug treatment. But the...

12 January 2021 144 1 View

Should I normalise data when measuring fluorescence intensity ?

I'm measuring fluorescence intensity of the product in an enzyme reaction in 37 celsius degree. I set a kinetic measurement in 1 hour. The fluorescence intensity of the BLANK ( contains only...

03 January 2021 5,554 5 View

Does CD31 indicate cell viability?

Hi, I am wondering if the immunofluorescent staining of CD31 antibodies can both mark endothelial cells and indicating their viability. In other words, if I perform immunofluorescent staining for...

23 December 2020 5,815 4 View

How to obtain clear Ru 3d5/2 peaks?

I am trying to obtain Ru 3d XPS spectra of samples with RuO2 content of 0.1 ~ 0.3%. But due to the overlapping of the C 1s region and maybe the low content as well, the Ru 3d5/2 peak was almost...

22 July 2020 7,789 4 View

Should EFA be applied for all items in the model or just within one variable?

For Data analysis using SPSS, EFA is used to indicate the number of factors. However, the input items for EFA analysis should be within one variable for each test or all items in the model?

19 April 2020 1,903 15 View

Valves for elemental mercury related experiments?

Hi, Does anyone know which type/brand of valve is usable in case of gas flow containing elemental mercury? Extra information: Our lab is working on a project related to mercury adsorption and...

17 June 2019 3,758 3 View

"Normal density of algae Thalassiosira pseudonana in mass production?

We are growing Thalassiosira pseudonana in 1,000L tank for shrimp larva in the hatchery. The maximum density gained is around 170,000 cells/mL. Does anyone have experience in growing this algae...

22 April 2018 508 4 View

How can I evaluate the reliability of the questionnaire?

08 July 2017 2,710 27 View

During TDM, Is it possible that the concentration of Tacrolimus keep being high over a period of several days without no drug administration?

I'm doing the population pharmacokinetics modelling with a Tacrolimus database and I found out that in some patients, there is a period of 5 days without administration, (for ex, at time after...

12 March 2017 1,704 17 View

Please help me to answer my question. What is the best commercial product for bacterial DNA extraction from milk?

bacterial DNA cow milk DNA extraction

21 February 2017 4,901 3 View

What are the harmful effects of these extreme rainfall on the health of newborns? What are the reasons for that?

Extreme rainfall associated with climate change is causing harm to babies in some of the most forgotten places.

01 March 2021 5,647 4 View

Is the box plot of climatic indices better illustrate from daily data or annual data?

Hello, I hope you have a good time. I work on a research project about temperature indices. Due to the high number of indices, I only work on tables and maps on an annual time scale. In other...

19 February 2021 9,742 2 View

What are the options for global scale hydrology DEM?

Hello, I am working on a global scale hydrology GIS project delineating global watersheds and doing some data analysis. I have in the past used hydro1K from the USGS that cover the entire world....

18 February 2021 5,109 5 View

What are the recently methods to estimate low-flow and high-flow time series in ungauged basins?

Hi Is there any new method to estimate ( predict ) low-flow and high-flow regimes in daily scales for ungauged hydrometric stations? Thanks

15 February 2021 3,493 3 View

Measurement of water retention in organic substrates without a pressure plate?

Hello, I'm trying to measure the physical and hydrological properties of organic substrate media. Currently, I'm referring The book Soil sampling and Methods of Analysis by Carter and Gregorich....

09 February 2021 3,233 8 View

Hye All, Greetings, How can i get surface run off water data?

this is for my thesis project, so this is the title of my thesis, the effect of land use on hydrological components in Muar, Johor, Malaysia any ideas, guidance, and suggestions all? Thank You, :)

02 February 2021 265 5 View

How to fix the voids on a floodplain map by using HEC_RAS model?

24 January 2021 9,054 2 View

How the precipitation Ratio and flow Ratio effect the output of the result?

I am doing HEC HMS calibration than i want to increase simulated value and volume of the computed so that my issue is how the precipitation and flow ratio make influential that result?

22 January 2021 152 2 View

Can anyone please tell me, what will be the Crop Coefficient value for shortleaf pine and oak tree in HEC-HMS model simulation?

my study area located in the forest region having Shortleaf Pine and Oaktree, thus in the Canopy Method I am trying to get the Crop Coefficient value of these trees but did not get anything.

18 January 2021 9,789 1 View

How can we determine the basin borders by GIS?

Hi We are working on a research about prediction river discharge in ungauged stations. we considered some stations and each of these stations has a sub basin that we know the area of each sub...

10 January 2021 3,806 5 View