Hi, I am trying to estimate total flavonoid content in crude extract, can you please provide any protocol to prepare catechin standard curve.
Thank you in advance.
Hi, you could prepare a calibration curve dissolving a defined concentration of catechin in 80 % MeOH .
Catechin is not the best for calibration curve as Philip noted above, rutin will give a better result. For the preparation dissolve 5 mg of catechin in 5 ml of analar methanol to obtain a stock concentration of 1mg/mL, then prepare 20, 40, 60, 80 and 100 microgram per mill. Use these concentrations to prepare the standard curve.
you can use this research
in this research we used Querciten
the protocol is one
Article Chemical Composition and Antioxidant Capacity of Eggplant Pa...
The standard catechin solution to be suck into pipette (0.25 ml, 0.5 ml, 0.75 ml, 1 ml, 1.25 ml, 1.5 ml, 1.75 ml & 2 ml) and transfer into respective conical flasks, labelled as 5 ppm, 10 ppm, 15 ppm, 20 ppm, 25 ppm, 30 ppm, 35 ppm, 40 ppm and 45ppm respectively. Then, conical flask filled with methanol until 1ml for each flask individually.
depends on the material you use, this was for MK
@ 0baid ullah what is the concentration of the stock solution
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