I am new to HPLC and will need to prepare a blank vial to wash in between running samples. What is the typical protocol for how to prepare a blank? Thanks in advance!
Chosen the washing solution is related to your sample and the mobile phase solution...
In general, chose washing solution that have the ablity to dissolve your analyte easily and in the same time being miscible with the mobile phase that you use
However, a needle wash (depending on the sample matrix) can be either the mobile phase (HPLC) or something stronger or weaker strength (assuming the sample matrix has other crap in it).