Help me to calculate quantity of salt for the preparation of 20mM phosphate
what pH you want to achieve? it depends on pH. see http://cshprotocols.cshlp.org/content/2006/1/pdb.rec8303.full?text_only=true for further help
I am performing a simple close range photogrammetry test and comparing outcomes of photogrammetry tools such as VisualSFM, Meshroom, Colmap etc. Kindly advice, what post evaluation tests/ criteria...
13 February 2021 6,410 3 View
In our current era there is a big confusion about the usage of G.652 and G.655 optical fiber cable. Theoretically G.655 is much better than G.652 but the operator feel that G.652 is giving almost...
11 February 2021 4,006 8 View
Various schools have emerged in the study of comparative literature. Mention the most prominent of them in a nutshell. And he talked about one of them?
09 February 2021 1,099 8 View
While reviewing technical articles, I encounter some papers where authors have used data balancing before splitting test and train (on full data), and some only balanced training data and let the...
04 February 2021 1,446 0 View
severity index, measuring method and procedure detailed
01 February 2021 1,160 11 View
The 1st image shows the moment when I import my design(IGES) into the Mechanical APDL. The 2nd image occurs when I wanted to Operate>Boolean>Divide Volume By Area. The design is a regular...
28 January 2021 2,962 3 View
Chronopharmacology is branch of biological science which deals with daily life, sleep and wake, day and night, feeding and fasting and other effects. we can manage many diseases including...
18 January 2021 9,045 3 View
Why there is no licensed vaccine available against congo fever? Besides what should be done to make the globally recognized Congo Vaccine?
11 January 2021 4,475 4 View
Every function of mind works in the brain. Study of divisions of mind for memory, thinking, analysis, conscious and subconscious mind is important for successful use of the mind.
10 January 2021 5,282 32 View
Hello everyone, I am aiming to write a PHD proposal of 1000 words. The research is described by the supervisor. That means, research questions, aim, and instruments are well defined by the...
01 January 2021 6,291 7 View
Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...
03 March 2021 1,499 2 View
Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...
03 March 2021 6,299 3 View
Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...
01 March 2021 2,215 2 View
Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.
01 March 2021 9,952 3 View
I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...
01 March 2021 9,015 2 View
I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...
26 February 2021 7,129 3 View
Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...
24 February 2021 5,749 3 View
Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...
23 February 2021 6,266 3 View
22 February 2021 9,932 11 View
I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...
19 February 2021 7,006 5 View