I am looking for a way to predict Transcription Start Site (TSS) in Aedes genome, Is there is any bioinformatics software or pipeline to predict TSS from genome sequence or a gene
To analyze Aedes genomes, DeepTSS (https://github.com/etirouthier/DeepTSS) may be trained.
I have attached with dataset
03 June 2022 7,880 0 View
LAMMPS software tutorials, workshop and study materials
20 October 2021 1,714 3 View
Our research team is involved in the study of drug loaded PLGA nanoparticles and nano lipid carriers (NLCs). We are planing to use Wyatt Heleos II MALS for particle size and PDI. Any insights/...
09 October 2020 5,099 4 View
Our team is trying to elucidate a reaction mechanism between X and Y. Both are organic compounds. What are different ways to approach to the problem. Your suggestion will be of great help.
20 September 2020 1,387 26 View
Any suggestion on the alternative methods for freeze drying PLGA Nanoparticles? Some articles reports the usage of liquid nitrogen followed by lyophilization. Will there be any effect of Liquid...
11 June 2020 5,801 3 View
Greetings, I am trying to isolate DNA from plant samples using CTAB method.After overnight step (icecold isopropnal) I could see the nice pellets over the bottom of the microtube . But during...
06 May 2020 8,447 0 View
If so, kindly suggest me the protocol used for Plant DNA pellet air drying through vaccum. At which stage should be followed? pressure, temp, time, microtip tupe (open/closed) etc. Please give...
06 May 2020 6,379 0 View
I wish to utilize cartosat DEM, publicly available form bhuvan site. However, I have analyzed and seen that the elevation values from cartosat DEM are very similar to ellipsoidal height of...
06 November 2018 4,945 3 View
My experiment is about understanding the cellular distribution of my protein with His tag. For this experiment, after experimental conditions i performed immuno-fluorescence staining of the...
23 July 2017 3,543 10 View
According to Kramer publication (Curr Protoc Mol Biol. PMC 2012 July 1) It is mentioned under "SL RT primer" Transfer to new tube, then overlay with 100 μL molecular-biology grade mineral oil....
14 April 2017 4,684 2 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...
03 August 2024 2,484 1 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
01 August 2024 1,514 1 View
I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...
28 July 2024 2,979 1 View
Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...
28 July 2024 8,953 1 View
Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...
28 July 2024 6,938 1 View