I am finding a lot of cellular debris in the culture flask? What are the reason behind it?

I am facing a problem that cellular debris observed in my SHSY5Y cells. I have seeded the cells in DMEM media with 10% FBS in T-25/ 60mm dish (antibiotic used PEN-Strepand gentamycin). I have also...

17 January 2021 3,074 3 View

How can we calculate theoretical stacking fault energy of unknown/new complex alloys?

Is there any method/theoretical approach to calculate the stacking fault energy of complex alloys such as high entropy alloys? The theoretical/ approximate value of SFE is needed for alloy design.

16 November 2020 7,074 3 View

How to fill the gap between two contigs?

Hi everyone, We have sequenced genome of data of a comparatively less studied species using Pacbio long read sequencer. Now after initial assembly we compared the contigs with previous version...

25 June 2020 3,442 3 View

How ultrasound and microwave can be effective factors on cotton and wool fabric which help the fabric to improve its dye uptake ability?

How ultrasound and microwave can be effective factors on cotton and wool fabric which help the fabric to improve its dye uptake ability?

15 June 2020 4,541 3 View

How COVID19 would affect the other research areas in Biology?

As the whole world is suffering with COVID19 infection; the research for vaccines are at its peak and so is the funding towards virology projects. Will it have a negative impact on funding in...

25 May 2020 8,579 3 View

How to make ROC plot for the validation of ligand-based-pharmacophore models in discovery studio?

Hello, I already made a ligand-based pharmacophore by utilizing 60 dataset compounds by catalyst module in discovery studio. But I want to explore the ROC curve of that pharmacophore in order to...

02 March 2020 9,044 5 View

How to solve the GROMACS error: The initial cell size (0.657461) is smaller than the cell size limit (0.683200)?

I am simulating a glycerol-water mixture system in a square box of length 3.5 nm. After the NPT step my box size decreased to 2.62984 nm. However, in the beginning of the production run step I...

27 February 2020 2,830 1 View

Can I import Abaqus simulated file to Ansys Workbench for further simulation?

I am using Abaqus/CAE for Laser Shock Processing (LSP), Can I import the simulated model file to Ansys Workbench for further analysis as I have to run two simulation for fatigue, FOD damage...

19 October 2019 7,031 2 View

Is there a proven way to reverse the cross- linking of long term formalin fixed tumors for western blotting?

Can anyone tell me if there is a way to reverse the cross-linking of long term formalin fixed tumors for protein work? Thanks

13 August 2019 1,168 2 View

How to reduce parasitics from layout?

Actually, I am doing layout in cadence, in pre-layout simulation I got gain around 80dB,but in post layout the gain reduce to around 70dB due to excessive parasitic capacitance and resistance. I...

26 April 2019 4,035 5 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

Microstrip antenna design tutorial of HFSS 3D layout module ?

I have designed a 24 GHz rectangular antenna on HFSS 3D model and got desired results while desing the same antenna with same dimensions I am getting resonance frequency at 27 GHz I am...

28 February 2021 4,426 2 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

Compact antenna for the frequency range 1 MHz-5 MHz?

Hello everybody! I have a problem with developing a compact antenna for the 1MHz-5MHz frequency range. Tell me what type of antenna can be used and what books and articles to read to perform this...

27 February 2021 7,710 4 View

Can impedance of antenna be less than 50 ohms?

I am simulating helix antenna in hfss, right now, giving excitation with 50 ohms port. When I see the results of Z parameter (real). It shows lower than 50 ohms. Do not understand, if it can be...

27 February 2021 4,218 7 View

Efficiency of Proximity Feed Patch Antenna ?

Greetings to all of You! I have searched some research papers for the designing of a proximity coupled patch antenna. Researchers are talking about the impedance bandwidth of the antenna but...

24 February 2021 7,662 3 View

How to generate AA6061 alloy or any aluminium alloy crystal in material studio?

I need to carry out molecular dynamics simulation on Aluminium alloy. For this i need the crystal structure that which could be further replicated to form a structure.

22 February 2021 1,410 1 View

2D or 3D phased array antennas??

Dear All, I will start my PhD maybe this year regarding phased array antennas. I'm interested in to find more detailed information about 3D phased array antennas and 2D phased array antennas. Then...

21 February 2021 368 3 View