How to perform SDS-PAGE for polysaccharide molecular weight distribution analysis?

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Greg Cote

Sorry, but SDS PAGE is not the best way for this. Polysaccharides are polydisperse, and will not give a sharp band on PAGE. In particular, the SDS will not bind in the same way that it binds to proteins, especially with a sulfated polysaccharide. I suggest you use HPSEC and/or light scattering for a determination of Mw and distribution.

Tjaard Pijning

Size distribution of oligo- and polysaccharides can be analyzed by HP-SEC. See e.g. this paper Article The Gram-negative bacterium Azotobacter chroococcum NCIMB 80...