Plasmid showing contamination of other components.
Dear Sir
I think the links below will benefit you in your search
http://vlab.amrita.edu/?sub=3&brch=186&sim=1097&cnt=1
http://vlab.amrita.edu/?sub=3&brch=186&sim=1097&cnt=2
https://academic.oup.com/femsre/article-pdf/1/3-4/149/18129375/1-3-4-149.pdf
Article Plasmid curing in bacteria
Best Regards
I am doing my Ph.D Research work for last 3 years with probiotic lactobacilli strains. I have worked with bacteriocin, plasmid, 16S rRNA gene sequencing and other criteria that satisfy the...
02 March 2018 5,257 6 View
I've find out sequence, but unable to construct phylogenetic tree of the same.
10 November 2016 1,522 2 View
I would also like to known, what % of gel I should use in SDS-PAGE to find out the proper band of bacteriocin. Whether any specification for staining and De-staining... Thanks in advance.
01 January 1970 2,136 6 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...
05 August 2024 1,573 4 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...
30 July 2024 6,648 1 View
I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?
29 July 2024 3,229 1 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View