How to perform plasmid curing experiment ?

Debashis Halder @Debashis_Halder8

08 August 2017 1 4K Report

Plasmid showing contamination of other components.

Osama Rahil

Dear Sir

I think the links below will benefit you in your search

http://vlab.amrita.edu/?sub=3&brch=186&sim=1097&cnt=1

http://vlab.amrita.edu/?sub=3&brch=186&sim=1097&cnt=2

https://academic.oup.com/femsre/article-pdf/1/3-4/149/18129375/1-3-4-149.pdf

Article Plasmid curing in bacteria

Best Regards

Badges
Science topic

More Debashis Halder's questions See All

Can anyone suggest me the free journals with good impact factor for publishing scientific experimental research with probiotic lactobacilli strains?

I am doing my Ph.D Research work for last 3 years with probiotic lactobacilli strains. I have worked with bacteriocin, plasmid, 16S rRNA gene sequencing and other criteria that satisfy the...

02 March 2018 5,160 6 View

How to creat an accurate Phylogenenetic tree of microbes using 16S rDNA sequence ? Is there any authentic software?

I've find out sequence, but unable to construct phylogenetic tree of the same.

10 November 2016 1,384 2 View

Can anyone provide me the most efficient protocol for isolation of bacteriocin form lactobacilli/lactic acid bacteria?

I would also like to known, what % of gel I should use in SDS-PAGE to find out the proper band of bacteriocin. Whether any specification for staining and De-staining... Thanks in advance.

01 January 1970 2,027 6 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View