I tried BIOXYTECHÒ LPO-586™ kit from OxisResearch. But I had to use a lot more cells than what is said in the protocol and had to treat cells with oxidative stress inducers to be able to get a readable data. Hope this helps. Good luck!

Thank you Ying Wang, I will take a look.

In brief; to 150 µl of sample the following was added: 1 ml trichloroacetic acid (TCA) 17.5%, 1 ml of 0.6% thiobarbituric acid , mixed well by vortex, incubated in boiling water bath for 15 minutes, then allowed it to be cooled ..

Then 1 ml of 70% TCA was added, and let the mixture to stand at room temperature for 20 minutes, centrifuged at 314 x g for 15 minutes, and the absorbance of the supernatant was measured at 532 nm.

Guidet, B., Shah, S. V.(1989). Am. J. Physiol. 257(26):F440.

Luveni Sonka