How to optimize the Lamp PCR assay?

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Abhijeet Singh

What do you mean by joining BSTs?

Michael Kim Jr Bonifacio Mendoza

Hi, for LAMP assay this is my protocol

first, I run my outer primer (F3 and B3) with either qPCR or conventional. If it works, then I'll proceed with the lamp assay.

For lamp assay, I used 1.6uM FIP, 1.6 uM BIP, 0.2 uM F3, 0.2 uM B3, 0.8 M LF, and 0.8M LB then used 8 units/uL of BST DNA Polymerase. You may try two assays in triplicates for BST 2.0 and BST 3.0 if which among the two is superior.

Eleni Makraki

join Bst 2.0 with Bst 3.0? what do you mean?!