Im doing real time PCR (probe-based) on samples containing much blood. I see my Ct values are quite much higher compared to samples containing no blood.

Ive seen in the litterature, that there are multiple things which can reduce the PCR effeciency when working with blood.

Does anyone have experience with simple tricks for increasing the effeciency? Polymerases which are more blood inhibition tolerant? Addition og pre treatment or helper reagents?

Im planning on doing the run again and a bit of BSA.