concentration of taqman probe is 10 umol，i want devise scheme to optimize dosage of Taqman probe.Total reaction volume is 20ul。
If you know the final concentration of the probe in the 20 uL reaction, you can figure out how many uLs to take from your 10 uM working stock. There's tools for that i.e.:
https://www.biosearchtech.com/support/tools/oligo-toolbox/oligo-dilution-calculator
Hello 朝阳 李 Li
For probe-based qPCR and RT-qPCR approaches, which use a labeled amplicon-specific probe to produce a fluorescent signal during enzymatic processing of the probe:amplicon hybrid, you should optimize the primer and probe concentrations for each primer/probe combination for optimal results. For gene expression analysis, you may also need to adjust these concentrations based on target abundance.
Optimal results may require titration of primers and probes or adjustement of the primer / probe ratio. The purpose of such a process is to determine the minimum amount of primers and probe required to obtain the most sensitive results with your assay.
Please refer to the link below. {See Primer titration matrix (Table 1) and Primer-probe ratio matrix (Table2)}.
https://www.eurogentec.com/assets/35def1bd-0c08-4f11-aace-d6245ee04950/res-tec-en-primer-probe-ratio.pdf
Best.
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