Anyone have a good protocol for extracting 4,4'-(1H-1,2,4-Triazol-1-ylmethylene)dibenzonitrile from Letrozole drug?

Can anyone give the detailed procedure for extraction.

30 December 2020 513 1 View

I am Looking For Spam Dataset ?

I am Looking For Spam Dataset? Can I find a comprehensive Dataset with.csv or .arff extensions which compatible with Weka?

03 December 2020 5,254 3 View

No title

08 November 2020 7,455 5 View

The second wave of the virus is Covid 20 !?

The second wave of the virus is Covid Does the virus develop and become more lethal and infect the lower part, i.e. the stomach and intestine, with severe diarrhea, intestinal bleeding and ulcers...

02 October 2020 4,767 1 View

How to discuss an insignificant p-value?

I have a small sample size (29 patients) and divided them into two groups each of which has a different sample size. I have an ordinal scale (dependant variable ) which is a scoring system to...

13 September 2020 7,298 31 View

What made English to become the language no. 1 in the world?

Why English in particular? Why not French, German, Arabic, Spanish, Chinese, or any other language?

27 July 2020 7,754 90 View

What is the most reliable method for performing ELISA test on mouse brain?

Dear fellow researchers, may I ask you for your advice regarding performing ELISA test on mouse brain? which method gives a better yield snap freezing the tissue in liquid nitrogen, storing it...

26 July 2020 1,376 3 View

How to calculate Stress intensity factor in ABAQUS XFEM with allowing for crack growth?

Dear All, I want to model crack growth in a plate using ABAQUS XFEM method. Here is my question about it: How to calculate Stress intensity factor in XFEM when crack growth in ON? At this time,...

17 July 2020 1,341 1 View

Scientific relationship between stress intensity factor (SIF) and crack tip opening displacement (CTOD)?

Dear connections , What is the scientific relationship between stress intensity factor (SIF) and crack tip opening displacement (CTOD) with specimen double edge cracked. So really I need your...

13 June 2020 9,426 1 View

How to keep in memory the global ecological pause during the COVID-19?

Dear Researchers, I would like to raise a debate with you, regarding the impact of COVID-19 in the environment and climate change. We are a group of researchers that have launched a map, which...

08 June 2020 9,286 3 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View