I am looking for suggestion regarding the isolation of Artemia ribosomes. I have isolated the ribosomes from the chromatographic purification but not sure about the color of the pellet for the Artemia ribosomes.
Are increasing the costs of disasters in the affected countries.
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The threshold voltage (Vth) of a MOS device plays a crucial role for its operation. At the same time, noise is an intrinsic factor. So how noise (flicker or thermal) change with the change in...
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If you don't understand my question let me know
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I am working on scale development in behavioral finance by undertaking a mixed-method approach using the exploratory sequential design. The phenomenon has diverse meanings in existing literature...
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I need to convert Available phosphorus into total phosphorus
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Bioinformatics tools like peptide cutter
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The burden of obesity is enormous. The greater challenge lies in accurately diagnosing obesity at the right time, reflecting the true increase in visceral fat. BMI may not accurately represent...
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#SEM #PLS-SEM #JASP #Smart-PLS
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How does high humidity affect the growth and development of crops?
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In what ways does high humidity impact the pollination process in agricultural plants?
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Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hello, I am searching for the binding position of a drug in a ribosome, based on previous work indicating it binds there. The resolution of the ribosome-drug Cryo-EM map is around 2.5 Å. I've been...
19 July 2024 2,155 0 View
According to standard guidelines, experts suggest proper feeding ratios to grow the ZF healthly.
15 June 2024 4,354 1 View
Feeding zebrafish larvae properly is a challenge if we need to grow them. According to standard guidelines, how many grams or milligrams of Artemia should be given to 6 dpf larvae?
13 June 2024 389 0 View
Hi, During biopanning rounds of phage display, after infecting TG1 cells with eluted phages and placing them on 2xTYGA I get inconsistent TG1 colony morphology. Some rounds the colonies are...
29 May 2024 8,097 0 View
Hi all-- I'm preparing plant root samples for RNAseq and have been investigating some samples that had suspicious bands in their lanes when I ran a 1% non-denaturing RNA QC gel. To spot check...
26 May 2024 7,139 1 View
I'm performing an antibody phage display with a VHH library and I consistently get frameshift mutants (mainly frame +2) after biopanning. I'm using TG-1 cells for amplification of phagemids and...
14 May 2024 412 2 View
I have used a column based kit for isolating the RNA from the insect and plant sample. I got a good quantity of RNA, but in the sample QC for Library preparation, the sample failed due to poor RIN...
06 February 2024 9,409 3 View
The only answer I have come close to is the "hidden break" event of ribosomal subunits reported in mollusks and insects. However, in these studies up to 3~4 bands are reported, in my case I have...
27 December 2023 1,121 0 View