I want to make a concentration of plant extract since I don't have any established data for my research which is C. roseus as antibacterial cream. Thank you and I hope you will help me. Our final defense is approaching but I still have issues in my paper.
Dear Scholar,
You will be using any positive control like known antibiotics also while performing either disc o well dilution studies. The MIC of the positive control can be traced out. So prepare the extracts in the following way.
01. 50 % less extract to the MIC of positive control
02. 25 % less extract to the MIC of positive control
03. Exactly equal quantity of the MIC of the positive control
04. 25 % more extract to the MIC of positive control
05. 50 % more extract to the MIC of positive control
From the results and if required you can fine tune the experiment again.
With regards,
Dr. Rajesh S Mony
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