I would like to measure fibrinogen in a 0.1g stool sample using an ELISA kit. How should I normalize my experiments?
1. Use intracellular housekeeping protein antibodies: select a relatively constant intracellular protein such as β-actin. Cells need to be lysed. How to design experiments to avoid non-specific binding of other endogenous proteins in cells to antibodies?
2. Use of extracellular housekeeping protein antibodies: How do I go about finding this protein that is consistently expressed in stool samples?
See the useful links:
Predicting disease course in ulcerative colitis using stool proteins identified through an aptamer-based screen. Nature Communications volume 12, Article number: 3989 (2021).
https://www.nature.com/articles/s41467-021-24235-0
Fibrinogen Activity Test. https://www.healthline.com/health/fibrinogen
Good luck
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