acridine orange and EtBr do different things Srinivasa. ETBr binds to DNA and then fluoresces under UV light allowing you to see where your dna has got to in the agarose gel after electrophoresis. Acridine orange is just an orange dye which you add in sucrose or glycerol solution to your dna sample and not to the gel. The glycerol makes it easier to load your dna sample into the well because the density holds it in the well. The orange dye lets you see that the loading is correct with no spill over into the next well.It also runs in the same direction as the dna in agarose but is smaller in size than bromophenol blue or xylene cyanol so is less likely to obscure your dna band and gives you a slightly better idea as to how far your dna has run within the gel

@Srinivasa Rao Palla:  even if the specialist knows that you wanted to name the dye....

would you - please - mind to correct (using the edit-function top right edge/margin to your question-form....)   the wrong / misspelled "according" in the title of your question to "acridine orange" (this honestly asked for better searchability in RG-archives....)  Thank you!