How to sample data to increase presence of solar panels in the images?

I am working on creating deep neural network on geospatial dataset and wanted to know how to do the sampling so that I can increase the presence of solar panels in the images. I cannot use entire...

25 February 2021 3,936 6 View

Do blood cells die after staining in blood smear analysis ?

This might be a very naive question but I've not been successful in finding sources that explicitly say that the cells are alive post staining. Do Romanowsky stains such as Giemsa, Wright etc....

09 September 2020 2,920 3 View

Is it ok if in measurement model one of the construct having less AVE than 0.5?

In measurement model, one of the construct having AVE 0.4 but the composite reliability is 1 and Chi square = 1.099, GFI = 0.995, CFI = 0.997, RMSEA = 0.022. So, is it OK to go ahead in SEM.

17 July 2020 7,088 4 View

Relative telomere length calculation with Ct values?

10 May 2020 7,398 2 View

In empirical research can we present two different framework?

In SEM, can we present two different models, one bases on theoretical base and other for hypothesis testing? OR In one model we have to present model fit and hypothesis?

03 May 2020 3,441 5 View

Which wavelet transform would be better to use for image classification?

I am trying to use wavelet transform as part of pooling operation in Convolutional Neural Network for Facial emotion classification.As part of this I tried with simple Haar wavelet but it doesn't...

05 February 2020 583 6 View

What is the lower limit for reliably counting cells in a typical hemocytometer ?

I am looking to quantify the counting errors associated with serial dilutions. Few sources mention a lower cap of 25,000 cells per ml - does this sound right ?

27 December 2019 3,714 4 View

Which are the perfect hardware platforms to compute ML-based inference at the network edge?

Edge Computing, IoT, Decentralized Machine Learning, Edge Gateways

12 October 2019 9,940 2 View

How to couple eulerian and lagrangian phases in abaqus?

Hello, I am trying to simulate airbind effect on door slamming. I am unable to couple lagrangian and eulerian sections. Thanks

10 September 2019 7,423 1 View

What are relevant theories that might help me to understand how people value urban open spaces?

Apart from economic values of urban spaces measured using CV and HP, there are other social and ecological benefits offered by urban open spaces. I want to understand whether urban residents...

06 September 2019 278 9 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

Can part of recombinant protein not be expressed?

Hello. I am currently trying to express a recombinant gene with Avitag at the N-terminal. I'm aiming at a final product that looks like this: Avitag-(target protein) The protein I use is already...

14 February 2021 2,039 4 View

Transformation into the Stbl3 chemo competent cells?

03 February 2021 1,111 5 View

What is the best way and technique for serial dilution of bactria to get accurate number of colony ?

Hello I am infecting cells with bacteria in a well and then I am doing serial dilution of this well 1 in 10 and culture the two last dilution on MHA agar I have difficulty in getting accurate...

26 January 2021 9,256 5 View

Good day, I have not been able to amplify the plasmid Frt-hspB1. Somebody could give an advice me?

I acquired the plasmid in Addgene, reconstituted the filter paper where the plasmid comes from with 30 microliters of TE buffer pH 8, immediately afterwards perform the transformation of E.coli...

25 January 2021 2,526 3 View

Has someone used TTC agar overlay method and can help me?

I have tried to use TTC agar overlay method for a few times to identify respiratory-deficient yeast which were affected by EthBr. The problem is that cells which should have stayed white also have...

22 January 2021 585 10 View

I am having trouble with cloning my gene. How can I fix this?

16 January 2021 6,566 8 View

What is the best way to differentiate L. rhamnosus and L. casei in plate co-cultivation?

I'm having troubles in the differentiation and quantitation of each bacteria population during co-cultivation. The morphologic characteristics are very similar and it's really difficult to count...

13 January 2021 1,550 1 View