5-ALA is a precursor of porphyrin, heme, and bile pigments, and it is metabolized into protoporphyrin IX (PpIX) in the course of heme synthesis. PpIX can accumulate into cancer cells and we then can use the absorption of PpIX for photodynamic therapy of cancers.
I have 5-ALA and I need a standard calibration curve with different concentration of 5-ALA but at the max absorbance wavelength of PpIX which is 630 nm. Is that even possible?
5-aminolevulinic acid does not have any absorbance at 630 nm, so you can use that wavelength to measure the concentration of PpIX in the presence of 5-aminolevulininc acid, but you can't use that wavelength to measure the concentration of 5-aminolevulinic acid at all.
As Adam Shapiro has mentioned, you can not measure the concentration of 5-ALA at 630 nm wavelength. A standard calibration curve of PpIX is required for finding out the concentration of PpIX synthesized by cancer cells when incubated with different concentration of ALA.
@ Mrinalini Sharma Thank you so much.
I will follow your instruction.
I want to measure the concentration of 5-aminolevulinic acid in the plant sample. But I do not have aminolevulinic acid for the standard curve. Is there an extinction coefficient for this material? Can I use this coefficient instead of the standard curve equation to determine the concentration of 5-aminolevulinic acid in the samples?