Hi everyone,
I need some help regarding how to carryout fluorescence measurements. I am a beginner in this area and I need someone to point me in the right direction. I am trying to prepare a composite of zinc oxide and a fluorescent dye (solvatochromic dye) for luminescent sensing applications. Our lab has a fluorolog-3 spectrofluorometer which has not been used for a long time and the solid sample holder is probably missing, although we hope to get one soon. But first, I need to be able to evalute the performance of my fluorescent composite with different solvents. Where I need help is this: how can I prepare my sample for fluorescent detection experiment? Do I suspend a known amount of the solid powder in each solvent and put it in a cuvette and measure? Or I suspend the powder in one solvent and added a calculated volume of other solvents one at a time for measurements? Thank you very much for your kind responses.
Dear Joe,
generally I recommend to you to order solid sample holder, which allows measurements at different angles or integrating sphere, which is suitable with to measure quantum yield if you have calibrated light source. If you have thin solid samples, you can use simple test. Put suitable piece of black paper into cuvette holder diagonally - 45 deg to incident beam and output beam. Then you can put you sample into holder and measure it in FF mode. If your sample isn't smooth and glossy then is also acceptable RA mode.
Thank you Prof. Michal Vik for taking time to answer and for giving kind suggestions.
Dear Joe Simeon,
For the trial, you can use anything that can hold a sample to become flat surface. If you need something in front of your sample (facing the incident beam) then use quartz glass which can transmit in UV region. Solid sample usually measured by "Front Face" method, please see attached file for the sample positioning suggestion. I don't recommend 45 degree angle since it is possible that your spectrometer doesn't have filter to cut strong reflection/scattering light. By the way, I also measured high concentrated liquid sample using front face method.
Sincerely.
Dear Dr. Dimas Firmanda Al Riza , thank you very much for your kind suggestions.
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