How to maximize the DNA yield from muscle and adipose tissue using QIAamp Kit?

05 May 2018 1 595 Report

I am using QIAamp Mini Kit for DNA extraction and having relatively low yields from heart, skeletal muscle and adipose tissue (around 2 ug). I tried to increase the amount of proteinase K and to extend the incubation time at 56 deg C to get complete tissue digestion but it didn't help much. Any suggestions?

Dario F. De Jesus

Have you used metal beads with proteinase K and dissolve well the tissues? What about trying the Gentra puregene kit? It doesn't have columns so...no material loss and you get high molecular weight DNA.

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