My surface swab samples are from paintings. I use DNeasy PowerSoil Pro DNA isolation kit (QIAGEN). My extractions are working but the yield is lower than 2ng/microL.
I imagine that you expect low values from a surface swab but the amount may be improved using any of this type of kit by heating the elution buffer to 70c and eluting with twice the recommended volume of the hot buffer in the final stage of purification
Article Method for improving the quality of genomic DNA obtained fro...
Dear Seka Senevirtne
Since you already used a well-known kit for DNA extraction, I suggest you increase the incubation time (before final spinning) by 10 minutes. I believe it will increase the concentration of the extracted DNA.
Feel free to put your query if it is not working.
Best,
Samrendra
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