Does anyone here have an e-copy of Handbook of Solvent Extraction by Lo, Baird and Hanson?

My qualifying exams are the next week and my university library is out of copies! I would greatly appreciate your help.

06 January 2021 9,929 1 View

How does Q5 GC enhancer works?

I'm doing a PCR with Q5 enzyme. I would like to know how Q5 GC enhancer works so that I can decide whether to add it or not.

11 February 2019 7,083 7 View

What buffer can replace the binding buffer in DNA purification kits?

I have a QIAquick spin column but do not have the PB and PE buffers provided with it. I do know however that the binding buffer needs to have a chaotropic salt. Is there any buffer/solution with...

04 June 2017 1,750 2 View

Can anyone share their experiences working with triple negative cell lines to screen for bioactivity?

Hi, I would like to hear suggestions about choosing cell lines for screening of small molecules. I understand that the common cell line used is MDAMB231. I also notice that use of MDAMB435 is...

09 February 2016 6,043 3 View

I am trying to run a flow cytometry experiment with MDAMB231 cells to do a cell cycle analysis. But why do I not see any cells when I run them?

06 August 2015 2,210 5 View

How can I obtain the band gap of the material based on the tauc plot if the absorption coefficient is unknown?

I have recorded the UV-absorption spectrum of my sample (bismuth titanate). I wish to know how we can determine the exact bandgap based on the tauc plot, if the absorption coefficient is unknown?

18 January 2015 2,901 3 View

When crossing mice of mixed FVB/C57Bl6 background to C57Bl6, at what generation of crossing to Bl6 would you expect a 100% black coat color?

See above

29 October 2014 8,909 3 View

How to differentiate between loading and doping of a semiconductor?

How do we differentiate the loading and doping in a semiconductor?

24 July 2014 7,501 1 View

Has anyone seen that there is GRP78 upregulation in the mRNA level but not in the protein level, in spite of GADD153 upregulation and XBP1 splicing?

I work with ER stress in primary cultures of cardiomyocytes. I have checked induction of GRP78 and other ER stress markers by both realtime PCR and western blotting. In spite of induction of...

23 February 2014 8,340 11 View

Can someone advise on primary antibody in milk?

I use TBS1X- Tween 0.1% for using primary antibodies for western blot. I had problems with one protein and a friend suggested me to try in 5% skimmed milk with sodium azide. The antibody worked...

04 July 2013 8,707 5 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

Discrete Phase model (DPM) particle break-up has particle size limits during particle tracking?

Hello, I am currently working on simulating water droplet partciles in a closed room. When I use uniform particles, without "particle break-up", I can carry out the simulation without any problem....

02 March 2021 3,053 2 View

How to size battery banks?

I am designing a small grid connected solar PV power-plant. As the battery's capacity is specified in Ah and demand in kWh. What is the best way to size the battery bank? I have battery price in...

28 February 2021 1,638 3 View

How to model the battery pack in PSIM or PSCAD?

Hello, I have simulated a single battery cell using Thevenin equivalent circuit. For a battery pack, I have a large number of cells connected in series and parallel connections. Is there any way...

28 February 2021 2,116 1 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View

High aspect ratio hexahedral mesh optimization solver?

I am interested in evaluating mesh optimization solvers that converge to high aspect ratio unstructured hexahedral grids. In other words, I am interested in a solver that optimizes warpage,...

26 February 2021 7,279 2 View

Zno nano particles colour changed from white to grey in process of calcination any suggestions?

In the formation of Zno nano particles process, after drying at 120 degree C, my zno nano particles are completely white but when i calcine it for 450 degree C it converts into grey colour. i...

26 February 2021 2,435 4 View