I want to make my own BL21 CodonPlus (DE3)-RIPL chemically competent cells, but I don't know whether the protocol is the same as the other strains (BL21 or BL21 (DE3)). Who can provide a detailed protocol for me?
The same protocol you would use for any other BL21 strain should be fine.
OK, thank you Benedik!I have tried using the CaCl2 method.
Calcium mediated transfection tends to be best for eukaryotic cells. You could always try lithium acetate.
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