I am not sure that I completely understand your question. Do you mean energy transfer? or do you mean energy level diagram modeling? You could specify the question in more delail. If so, I believe some Experts in the field will offer you good suggestions.
You can load the handypea data in biolyzer software and it will calculate all of the jip test parameters and will draw the pipeline models for you. You need to define one fluorescence curve as a refrence to compare all other samples with.please see web site.
BTW a quick question: I've tried to contact the Biolyzer developers and either by mail and website but without any success.. How can one get this software?