How to make a fasta file with a conjugated peptide?

25 March 2019 0 3K Report

Does anyone know how to make a fasta file with a conjugated peptide? I've only done this with amino acids but i have to also add the small molecule that is conjugated to the lysine mid sequence. or make a proteinfile "sequence" in Xcalibur's protein calculator program?

Badges
Science topic

More Charity Bechtel's questions See All

Effect of flipped blended learning on secondary school students in biology ?

I need help on this topic

19 June 2024 9,365 3 View

Can someone advice: LTQ Orbi XL - repeated cal failure in the trap - neg mode ion electron multiplier gain?

I'm having trouble calibrating my LTQ Orbi XL. All positive and negative calibration/checks passed accept in the Trap in negative mode, the ion electron mult gain. It failed bc of the gain match...

03 January 2019 4,727 2 View

How do you eliminate orbitrap electronic noise m/z peaks?

I have 2 m/z peaks that will not go away. not solvent/column/online or offline they are always there. I was told they are prob electronic noise peaks. Does anyone know how to isolate the...

20 December 2018 5,274 2 View

Potential monitoring system for shakers in CO2 incubators?

We are looking for a monitoring system like this for our plate shakers in our CO2 incubators: https://www.thermofisher.com/order/catalog/product/SMARTKIT1 One of our shakers failed during the...

25 August 2017 1,165 1 View

How can exchange rate affects an economy

Exchange rate affects an economy because it has a direct link to the currency of the nation and can determine the economic condition of the nation involved.

15 April 2017 9,302 8 View

I am using a retrospective cohort of all infant deaths. Should I be doing a case control study instead?

In this cohort I have 11 abusive deaths and 170 non abusive deaths

03 March 2017 8,788 8 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

What are the steps of formulating a herbal extract into a sheep/goat drench?

I am working on natural remedies for de-worming in small ruminant livestocks. Part of my project involves formulation of drench-type product, which I have not much experience in. My experience is...

30 July 2024 9,806 1 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View

Can we convert a thousand of FASTA sequence in numeric form in .csv format? If yes kindly send me the script for the same?

I have a .text file for various FASTA sequence , and i want to convert these sequences into a numeric file which will be in .csv format. OR I want to extract physiochemical properties(features)...

25 July 2024 3,650 2 View

Has the Affordable Connectivity Program Helped You/Your Community? And Would Bulk Tablets on a Similar Program Help Your Business?

Do You Believe VP Harris Would Show Empathy to Tech Companies in Her Administration as POTUS? I ask in light of the fact that, the ACP was not re-funded this round. So many and businesses need...

24 July 2024 4,732 3 View

What does the difference \lambda_j - \lambda_{j+1} represent in relation to the conjugate partition of \lambda?

For a partition of an integer \lambda, what does the difference \lambda_j - \lambda_{j+1} represent in relation to the conjugate partition, , with \lambda_j being the j-th element of \lambda?

23 July 2024 8,008 1 View

Mass spectra averaging algorithm?

I am now developing a python module for ms2 database searching, would like to realize a function that similar to what Xcalibur did, choose multiple mass spectra and get an averaged spectra. But...

22 July 2024 3,975 1 View

Is it possible to conjugate an NHS ester to the N-terminus of a peptide ON RESIN?

So i have a few peptides (synthesized by SPPS and not cleaved from resin) and I want to add a maleimide label. We have NHS-PEG-Maleimide and from literature it is evident that the free amine group...

18 July 2024 4,075 3 View

how does the mass spec fraternity handles peptide map of PEGylated proteins ?

I would like to do peptide map of a protein which is attached to PEG via lysines. Now the best approach would be to de-PEGylate it but then do the peptide map. But there is little literature on...

14 July 2024 5,634 2 View

Does anybody have Anti-Claudin8 and Anti-Claudin2 Primary conjugated Ab (Target Species: Human)?

I need Anti-Claudin8 Primary and Anti-Claudin2 conjugated Ab for my immunofluorescence experiment. If anybody has worked on it. Please share the accession number/details.

14 July 2024 485 0 View