Charity Bechtel

4 Questions 3 Answers 0 Followers

Questions related from Charity Bechtel

How to make a fasta file with a conjugated peptide?

Does anyone know how to make a fasta file with a conjugated peptide? I've only done this with amino acids but i have to also add the small molecule that is conjugated to the lysine mid sequence....

25 March 2019 3,063 0 View

Can someone advice: LTQ Orbi XL - repeated cal failure in the trap - neg mode ion electron multiplier gain?

I'm having trouble calibrating my LTQ Orbi XL. All positive and negative calibration/checks passed accept in the Trap in negative mode, the ion electron mult gain. It failed bc of the gain match...

04 January 2019 4,765 2 View

How do you eliminate orbitrap electronic noise m/z peaks?

I have 2 m/z peaks that will not go away. not solvent/column/online or offline they are always there. I was told they are prob electronic noise peaks. Does anyone know how to isolate the...

21 December 2018 5,306 2 View

How accurate should MSMS HCD Orbi fragments be?

How accurate should MS2 data be? fragmented by HCD in the Orbi? My precursor ions 1300+3 - 800+5m/z, are accurate to xxxx.xx. The molecule is 4000 Da intact MS work, top down no digest.

27 March 2018 724 2 View