Hello,

I am in the process of cartilage and bone staining some tadpole samples. I am currently between steps right now (just finished alcian blue stain and dehydration step). I’m about to start the clearing step prior to bone staining, however when making the 1% trypsin enzyme solution 1g trypsin powder, 30 mL aq sodium tetraborate (I use borax and make a solution of that on the side first), and 70ml of distilled H2O), I noticed after gentle stirring that there is a lot of left over residue and some "stringy floaters" in the solution. I’ve attached a photo for reference.

Should I be mixing the solution more? Should I sterile filter it before use? I want to ensure the solution is correct before continuing the protocol.

Thank you!