How to maintain an anaerobic environment for bacterial growth?

04 April 2013 1 7K Report

Using Nitrogen gas and monitoring by Resazurin is not working well, since it remains red instead of yellow.

You may consider adding reducing substances such as cystein-HCl and/or sodium sulfide (e.g. 0.5 g / L each). It works very well for culturing methanogens.

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