The method I have for isolating acidophiles from mine tailing include the addition of iron sulfate or sulfur for enrichment before isolation (9K media, (NH4)2SO43g, KCl 0.1g, K2HPO4 0.5 g,

MgSO4.7H2O 0.5 g, Ca (NO3)2 0.05 g). But I want to know if I will still be able to isolate acidophiles from the mine tailing using 9k media without the addition of iron sulfate or sulfur. The idea is to isolate different species.