Anyone who used chelating agents during DNA extraction?

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Ijaz Durrani

DR Rosina ---kindly have a looks at the following useful links:

https://academicjournals.org/article/article1379766796_Khosravinia%20%20and%20%20Ramesha.pdf

DNA extraction from individual larvae, nymphs, adults, or ovaries was accomplished using the Genomic DNA from Tissue kit (Macherey–Nagel). The presence of S. pierantonius DNA was quantified by amplifying the sequence for the S. pierantonius-specific gene, nuoCD. Real-time PCRs were normalized to the host’s β actin gene. The technique of real-time PCR uses fluorescent probes to quantify the amount of double-stranded DNA. While standard PCRs only look at the amplified product at the end of a reaction, real-time PCR measures the formation of that product over time.

https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/dna-extraction

Regards--

Obinna T. Ezeokoli

Hi Rosina Nkuna

You can try using Chelex 100. See Chapter DNA Extraction for PCR: Phenol/Chloroform vs. Chelex — A Com...

https://www.bio-rad.com/en-za/product/chelex-100-resin?ID=6448ab3e-b96a-4162-9124-7b7d2330288e

Article A Simple, Efficient and Rapid Method for Good Quality DNA Ex...